Table 2 of
Smith, Mol Vis 2014; 20:1328-1356. Table 2 of
Smith, Mol Vis 2014; 20:1328-1356.
Table 2. Acetylcholine receptor primer pairs.
| Subunit | Accession number | Primer (5′-3′) | Product (bp) | Temp (C°) | Primer ratio (nmol) | Product homology to mouse sequence |
|---|---|---|---|---|---|---|
| α2 nAChR | NM_144803 | F- GTGCCCAACACTTCCGATG | 126 | 55.8 | F400:R600 | 92% |
| R-TGTAGTCATTCCATTCCTGCTTT | ||||||
| α3 nAChR | NM_145129 | F- CCAGTTTGAGGTGTCTATGTC | 198 | 55.8 | F300:R200 | 99% |
| R- TCGGCGTTGTTGTAAAGC | ||||||
| α4 nAChR | NM_015730 | F-CTCAGATGTGGTCCTTGTC | 178 | 62.6 | F500:R400 | 95% |
| R- GAGTTCAGATGGGATGCG | ||||||
| α5 nAChR | NM_176844 | F- CATCGTTTTGTTTGATAATGC | 90 | 55.8 | F500:R300 | 84% |
| R- TGCGTCCAAGTGACAGTG | ||||||
| α6 nAChR | NM_021369 | F- TGTCTCCGATCCCGTCAC | 213 | 62.6 | F600:R500 | 98% |
| R TTGTTATACAGAACGATGTCAGG | ||||||
| α7 nAChR | NM_007390 | F-GGTCATTTGCCCACTCTG | 130 | 57.6 | F500:R500 | 99% |
| R- GACAGCCTATCGGGTGAG | ||||||
| α9 nAChR | NM_001081104 | F- ACAAGGCCACCAACTCCA | 152 | 54.0 | F400:R400 | 81% |
| R- ACCAACCCACTCCTCCTCTT | ||||||
| α10 nAChR | NM_001081424 | F- TCTGACCTCACAACCCACAA | 168 | 54.0 | F500:R400 | 94% |
| R- TCCTGTCTCAGCCTCCATGT | ||||||
| β2 nAChR | NM_009602 | F-CCGGCAAGAAGCCGGGACCT | 152 | 62.6 | F300:R400 | 97% |
| R- CTCGCTGACACAAGGGCTGCG | ||||||
| β3 nAChR | NM_173212 | F- AAGAAGCAGACTCCTACC | 123 | 62.6 | F200:R600 | 90% |
| R- AACAACCTGACTGATGAAG | ||||||
| β4 nAChR | NM_148944 | F- CTACAGGAAGCATTAGAGG | 146 | 50.0 | F300:R200 | 84% |
| R- CAGAATACACACAATCACG | ||||||
| m1 mAChR | NM_007698 | F-GACCCTACAGACCCCTCTCC | 165 | 66.2 | F600:R500 | 93% |
| R- CCCTTCCTCCAGTCACAAGA | ||||||
| m2 mAChR | NM_203491 | F-CGGCTTTCTATCTGCCTGTC | 169 | 50.0 | F100:R400 | 96% |
| R- GGCATGTTGTTGTTGTTTGG | ||||||
| m3 mAChR | NM_033269 | F-GTACAACCTCGCCTTTGTTTCC | 244 | 62.6 | F400:R200 | 99% |
| R- GACAAGGATGTTGCCGATGATG | ||||||
| m4 mAChR | NM_007699 | F-GCCTTCATCCTCACCTGGAC | 146 | 62.6 | F400:R200 | 99% |
| R- AGTGGCATTGCAGAGTGCAT | ||||||
| m5 mAChR | NM_205783 | F- CCATGGACTGTGGGAAGTCA | 215 | 62.6 | F300:R200 | 99% |
| R- CAGCGTCCCATGAGGATGTA | ||||||
| RyR3 | NM_177652 | F- AAGGTCATACTCCATCAGG | 110 | 50.0 | F400:R200 | 98% |
| R- AATAAGGCGGTTGTGTTC |