Figure 8. Wholemount choline acetyltransferase (ChAT) immunohistochemistry (IHC). There was a significant increase in the number of ChAT immunoreactive amacrine cells in the inner nuclear layer (INL) compared to the GCL in both A: WT C57BL/6J mice, *p<0.05 (n=5) and B: α7 nAChR KO mice, **p<0.001 (n=5). Error bars represent SEM. There was no significant difference between WT and α7 nAChR KO mice. For each retina, cell counts were obtained from five optical z-stack confocal sections taken under 40X magnification in the INL and the GCL, respectively. Regions of interest were non-overlapping 500 μm by 500 μm areas approximately 500 μm away from the optic nerve. Scale bar, 50 μm.