Figure 7. Western blot analyses with antibodies against acetylcholine receptors. Protein extracts from WT C57BL/6J mouse retina. A: Western blots obtained had a single band at the predicted molecular weight. This confirmed the presence of the nAChR subunit
and mAChR subtype protein and antibody specificity. Blots for each antibody were obtained separately and compiled for the
figure. α1, α3, α5 (AB24719-Rat) 51 kDa; α9 nAChR (AB49065-Rb), 53 kDa; m1 mAChR (AB5164-Rb), 60 kDa; m2 mAChR (mAB367-Rat),
52 kDa. B: Representative western blots for control conditions; clean, with no bands present. IgG: Immunoglobulin G; Rb: Rabbit.