Figure 5. A comparison of significant qPCR targets across experiments. The α2 nAChR subunits were upregulated in the whole retina and downregulated in the GCL. The α5 nAChR subunits were downregulated in the whole retina using either superarray (SA) or designed primers. The α9 nAChR subunits were upregulated in the whole retina and downregulated in the iINL. The α10 nAChR subunits were upregulated in the whole retina. The β2 nAChR subunits were upregulated in the oINL. The β3 nAChR subunits were downregulated in the iINL. The β4 nAChR subunits were upregulated in the whole retina and the oINL and downregulated in the iINL and the GCL. The m1 mAChR subtypes were upregulated in the whole retina using designed primers and downregulated using SA primers. The m2 mAChR subtypes were upregulated in the GCL and downregulated in the iINL. The m4 mAChR subtypes were upregulated in the whole retina, the oINL, and the GCL and downregulated in the iINL. SA- SuperArray, WR- whole retina, oINL- outer portion of INL, iINL- inner portion of INL, GCL- ganglion cell layer, Error bars represent SEM. ***p<0.001, SA (n=8), WR (n=11), LCM (n=11).