Table 1 of
Chen, Mol Vis 2014; 20:132-139. Table 1 of
Chen, Mol Vis 2014; 20:132-139.
Table 1. Primers used in sequencing of the RS1 gene and allele-specific PCR analysis.
| Exon/mutation site | Primer sequence (5′-3′) | Size (bp) | TM (°C) |
|---|---|---|---|
| 1 | F:TCTTCCATGAGACTTCCTTGTTGA | 485 | 60 |
| R:AGATTTCTGAGACCCATCCTGTT | |||
| 2 | F:TCTTCCATGAGACTTCCTTGTTGA | 249 | 58 |
| R:TACATTTAAAAACAAAGTGATAGTCCTC | |||
| 3 | F:CCAGGGTGGCAGACATTTT | 406 | 60 |
| R:GGTAGCGTTCAGGGGGTT | |||
| 4 | F:TTCCTTTACTTCATCCTTCATTCC | 550 | 60 |
| R:CTCACTGTAACCTCCGCTTCC | |||
| 5 | F:GCAGGGAGAGGGAGAATGAGA | 570 | 60 |
| R:CCAAAGCAAGCCCAGGAA | |||
| 6 | F:CAGTTCCAGATGTCCCAAGCA | 432 | 62 |
| R:TGTCCATCTCGGTGGTGTGTG | |||
| 5/p.Y151H | R: TCCTGTACTGCACGCTGaG | 347 | 60 |
| 5/p.Y166H | R:TTCCAGTCTGGTCCTTGaG | 392 | 60 |
| 5/p.K167E | R:GTTGTTTCCAGTCTGGTCCaC | 397 | 60 |
| 6/p.I194N | R:CGGATGAAGCGGGAGATGT | 271 | 62 |