Figure 2. Vsx1+/+ and Vsx1−/− mice do not respond to FD. A: Vsx1+/+ animals were unresponsive to goggling and showed a similar refractive myopic shift (goggled minus opposite eye) as their age-matched non-goggled controls across 4 weeks of form deprivation (FD) (two-way repeated-measures ANOVA treatment-age interaction, F(4,65)=0.131, p=0.97). B: Vsx1−/− mice did not respond to FD and showed no change in refractive error across the goggling period (two-way repeated-measures ANOVA, F(4,65)=0.609, p=0.658). C: C57BL/6J mice showed a significant myopic shift in the goggled animals compared to the naïve controls at 4 weeks after FD treatment (two-way repeated-measures ANOVA, F(4,72)=3.085, p=0.024). Holm-Sidak post-hoc comparisons ***p<0.001. Data are expressed as the mean ± standard error of the mean (SEM).