Figure 6. The TGF-β concentration in Sema3A-treated HUVECs. The concentration of free TGF-β1 in the supernatant was measured using an ELISA kit. The statistical analysis of the free TGF-β measured with ELISA at 48 h is presented. Sema3A inhibited the response of TGF-β. Each experiment was performed in six wells and was repeated at least three times. The data are presented as the means±SEM. **p<0.01; ***p<0.0001.