Appendix 1 of Fan, Mol Vis 2014; 20:117-124.
To access the data, click or select the words “Appendix 1.” A) Recombinant CRYAA protein was analyzed by 10% SDS–PAGE with Coomassie brilliant blue staining. B) Recombinant CRYAA protein was analyzed by western blotting with anti-alpha A Crystallin antibody. Molecular weight of recombinant CRYAA is 19.9 kDa. M indicates the molecular mass markers. Lanes 1, 2, and 3 indicate 50ng, 25ng, and 12.5ng of CRYAA, respectively.