Figure 5. Murine cytomegalovirus infection, autophagy, and mammalian target of rapamycin pathway. Retinal pigment epithelial (RPE) cells were infected with murine cytomegalovirus (MCMV) at low multiplicity of infection (MOI) = 1 in normal medium or in medium containing rapamycin (10⁻⁶ M). Samples were collected at day 2 (A) and 3 p.i. (E). Expression of processed light-chain 3B (LC3B), P-mammalian target of rapamycin (mTOR), mTOR, P-P70S6K, and P70S6K was monitored and quantified at day 2 (B–D) and at day 3 (F–H). Rapa: rapamycin; 2d: 2 days postinfection; 3d: 3 days postinfection. *p<0.05, **p<0.01, ***p<0.001, ANOVA. Data are shown as mean±SEM (n=3).