Figure 2. Autophagic flux during murine cytomegalovirus infection of retinal pigment epithelial (RPE) cells. RPE cells were infected with murine cytomegalovirus (MCMV) at low multiplicity of infection (MOI) = 1 in normal medium or in medium containing chloroquine (CQ, 10⁻⁶ M) or ammonium chloride (NH₄Cl, 10⁻⁶ M) to block autophagic flux for 6 h (A), 12 h (B), 24 h (C), 2 days (D), and 3 days (E). Expression of processed light-chain 3B (LC3B) was monitored.