Figure 5. Fluorescence immunostaining of human epiretinal membranes (ERMs) in negative controls. No immunostaining was observed in negative controls (arrow), which were performed by substituting the primary antiserum with phosphate buffered saline. Secondary antibodies were used cyanogen (CY) 3-conjugated goat anti-rabbit-fluorescein isothiocyanate (A) and FITC-conjugated goat anti-mouse-tetramethyl rhodamine isothiocyanate (B). Nuclei were detected using DAPI. Merged images (C), DAPI (blue). Scale bar represents 100 μm.