Table 3 of
Dharmarajan, Mol Vis 2014; 20:1085-1108. Table 3 of
Dharmarajan, Mol Vis 2014; 20:1085-1108.
Table 3. List of HUMAN primers used in qPCR.
| Gene | Accession number | Primer | Sequence | Product length | Efficiency (%) |
|---|---|---|---|---|---|
| Gfap2 | NM_002055.4 | Forward | GCACGCAGTATGAGGCAATG | 139 | 97.35 |
| Reverse | TAGTCGTTGGCTTCGTGCTT | ||||
| Pax2 | NM_003990.3 | Forward | CCAGTTGTGACTGGTCGTGA | 206 | 107.14 |
| Reverse | GGCATTAGTAAGGCGGGGTT | ||||
| S100-Β | NM_006272.2 | Forward | GACCAGGAAGGGGTGAGACA | 141 | 105.74 |
| Reverse | TGATGAGCTCCTTCAGTTCGG | ||||
| Vim | NM_003380.3 | Forward | GCAGGATTTCTCTGCCTCTTC | 198 | 103.251 |
| Reverse | CTGCACTGAGTGTGTGCAATTT | ||||
| Gs | NM_002065.5 | Forward | CTTAACCCACCAACCTGCCTG | 221 | 105.48 |
| Reverse | AGGTGGTCATGGTGGAAGGT | ||||
| Egfr | NM_005228.3 | Forward | AAACAACACCCTGGTCTGGA | 126 | 95.85 |
| Reverse | GGGATCTTAGGCCCATTCGT | ||||
| Tlr-4 | NM_138554.4 | Forward | GTGAGACCAGAAAGCTGGGA | 151 | 105.57 |
| Reverse | TGCCTAAATGCCTCAGGGGA | ||||
| Pcan | NM_002851.2 | Forward | TCCACAGATTTCAGTTTTGCAG | 198 | 98.81 |
| Reverse | CCCCTCAGCTAGACCAATACG | ||||
| Ncan | NM_004386.2 | Forward | GAGGTGCACTCAGATCCCTG | 196 | 96.51 |
| Reverse | GGCAGACAAAGCCATTGACC | ||||
| Timp2 | NM_003255.4 | Forward | GGCGTTTTGCAATGCAGATG | 72 | 105.47 |
| Reverse | TCGTTTCCAGAGTCCACTTCC | ||||
| Mmp9 | NM_004994.2 | Forward | CGACGTCTTCCAGTACCGA | 81 | 97.12 |
| Reverse | TTCAACTCACTCCGGGAACTC | ||||
| Mmp11 | NM_005940.3 | Forward | CTGGGAGAAGACGGACCTCA | 242 | 105.26 |
| Reverse | TCTTGGGGAAGAAGGCATGG | ||||
| Mmp14 | NM_004995.3 | Forward | GTGGTCTCGGACCATGTCTC | 143 | 92.26 |
| Reverse | AGCCATATTGCTGTAGCCAGG | ||||
| Glast | NM_004172.4 | Forward | GGCTAGCCTGCCTGCTTAC | 165 | 105.29 |
| Reverse | TGTCTGGGAATCACCCACAG | ||||
| Ednrb | NM_001201397.1 | Forward | TGCTTGCTTCATCCCGTTCA | 197 | 105.82 |
| Reverse | TCCCGTCTCTGCTTTAGGTG | ||||
| Lcn2 | NM_005564.3 | Forward | GACCCGCAAAAGATGTATGCC | 197 | 99.22 |
| Reverse | CTCACCACTCGGACGAGGTA | ||||
| Asic1 | NM_020039.3 | Forward | CGCAGCTTCAAACCCAAACC | 169 | 105.85 |
| Reverse | GGCCCGAGTTGAACGTGTA | ||||
| Kir2.1 | NM_000891.2 | Forward | ACTCTCGTCGGACCCTCC | 183 | 94.54 |
| Reverse | GCGACTTGTTGCTCAGGTTG | ||||
| Spp1 | NM_001251830.1 | Forward | AGCCAAACAACAAATGGGCA | 199 | 98.46 |
| Reverse | AGATGGGTCAGGGTTTAGCC | ||||
| Txnip | NM_006472.4 | Forward | TCAAGATGCCGAACCCTGTG | 130 | 96.45 |
| Reverse | AGTGAGGGGCCAATATCCCT | ||||
| Gal3 | NM_002306.3 | Forward | GCCTTCCACTTTAACCCACG | 173 | 105.55 |
| Reverse | ACTGCAACCTTGAAGTGGTCA | ||||
| β2-Microglobulin | NM_004048.2 | Forward | AGATGAGTATGCCTGCCGTG | 120 | 102.37 |
| Reverse | TCATCCAATCCAAATGCGGC |