Table 2 of
Lee, Mol Vis 2014; 20:1037-1047. Table 2 of
Lee, Mol Vis 2014; 20:1037-1047.
Table 2. Primer sequences for bisulfite sequencing or pyrosequencing.
| Genes | Method | Sequences (5’-3’) | Annealing Tm (°C) | Product size (bp) |
|---|---|---|---|---|
| DUSP22 (1st site) | bisulfite sequencing | F: GGGGAGTTTTTAGAGATTAGGTTTTT R: AATCTCCAAATCCCCCTTTAAC | 71 | 131 |
| DUSP22 (2nd site) | bisulfite sequencing | F: GTATAGAAAGTTTTGTTTTTTA R: TATTCATCCCATTCCCCATAATA | 81 | 226 |
| IRAK1 | pyrosequencing | F: TTAAATGAGGGTTGGGGTAGTAGTAA R: ACAACAACCTTAAACCATTCAATCTC | 69 | 109 |
| CXCL6 | pyrosequencing | F: GGTTATTGGAGAGGAGGAGTATTT R: CAACAAAATCTCATCCCCTAAACTTA | 68 | 95 |