Table 1 of
Wang, Mol Vis 1996;
2:3.Table 1 of
Wang, Mol Vis 1996;
2:3.
Table 1. Sequence and location of primers used for PCR
Primers (PDEA-1 to PDEA-4) used for RT-PCR correspond to the human (h) PDEalpha cDNA sequence, selected from the consensus region in different species. All other PDEA primers correspond to canine (c) PDEalpha cDNA sequence. Location of all the PDEA primers are shown with respect to cPDEalpha cDNA sequence. Nucleotides (shown in lower case) in some primers have mismatches with the canine sequence because those primers were selected either from the human sequence, or from preliminary canine sequence. Phagemid vector (pBK-CMV) specific primers were selected either from the multiple cloning site or from the flanking region. The bold-italicized region of primer pBK-V represents the linker used to make the canine cDNA library.
Primer sequence(5'to3') Source of Primer Name of primer Location -------------------------- ---------------- -------------- --------- gCTTTGCCAAACAGTACTACAACC hPDEalpha cDNA PDEA1 192-215 AgTTGTGcAGGTAACTCAGGTG hPDEalpha cDNA PDEA2 815-836 GGCCCTGGTGCGgTTC hPDEalpha cDNA PDEA3 1759-1774 ATGGGATTCTGtTGCAGCAC hPDEalpha cDNA PDEA4 2393-2412 TTCAACGTGGGGCAGACCAT cPDEalpha cDNA PDEA5 1832-1851 CACTACGTCCTTCCCATTCATTATGG cPDEalpha cDNA PDEA6 681-706 GTCATaAAGAAGCTGTGCTTCCTCC cPDEalpha cDNA PDEA7 380-404 GTCATGGGTGAGGTGACAGCAGAG cPDEalpha cDNA PDEA9 137-160 cCAACGTTTTGCCGAACTCCAAG cPDEalpha cDNA PDEA11 2032-2054 TCCACCCTATTCTGGTCCCA cPDEalpha cDNA PDEA16 1036-1055 ATGGTCTGCCCCACGTTGAAGCC cPDEalpha cDNA PDEA17 1829-1851 TTGCTTGGCTGTCTGCTGCTT cPDEalpha cDNA PDEA18 2624-2644 GCAGGTCGACACTAGTGGATCC pBKCMV pBKII 1092-1113 CCGCTCTAGAAGTACTCTCGAGTT pBKCMV pBKV 1052-1067 CGACTCACTATAGGGCGAATT pBKCMV T7 980-1000 |