Molecular Vision: Giovingo, Mol Vis 2013; 19:2151-2164. Table 1

Table 1. Western immunoblot evaluation of annexin 2 and caveolin expression in cultured human TM cells and microdissected porcine TM tissues

Protein	Function	Human TM Cells*			Mircodissected perfused porcine TM*
Protein	Function	Control (PBS)	sCD44 treatedwith 0.1 ng for 24 h	Ratio	Control (DMEM)	sCD44 treated with 1 ng for 72 h	Ratio
Annexin 2	Junctional Assembly Actin Cytoskeleton Chloride Channel	109	69.2	0.63	90.6	48	0.53
Annexin 2 phosphorylated	Negative modulator of actin assembly	11	41.1	3.72	47.9	56.8	1.19
Caveolin 1	Oligomers form lipid rafts	51.4	60.2	1.17	19.9	86.2	4.34
Caveolin 2	Lipid rafts	25.6	24.2	0.95	100	89.1	0.89

Protein loading control for each western blot was GAPDH and the protein abundance is expressed as densitometric units and normalized to Jurkat cell lysate as a positive control. *in 100,000s of densitometric units annexin 2 and caveolin 2 showed decreased concentrations in human and porcine TM cells when samples were treated with sCD44. Phosphorylated annexin 2 and caveolin 1 concentrations were increased in human and porcine TM cells when treated with sCD44. Comparison of densitometric data are an estimate of relative changes in protein concentration.