Figure 3. Effects of Ad-sCD44 and Ad-CD44S transduction on CD44 levels on day 7 in human trabecular meshwork cells. CD44 was determined by western blots of culture media and lysates of trabecular meshwork cells. The lane numbers signify the following: Lane 1, Ad-sCD44 media; lane 2, Ad-sCD44 cell lysate; lane 3, Ad-CD44s media; lane 4, Ad-CD44s cell lysate; lane5, Ad-empty media; lane 6 Ad-empty cell lysate; lane 7, fetal bovine serum (FBS) control; and lane 8, isolated 32-kDa sCD44. Western blots were performed in triplicate.