Figure 2. Effects of Ad-sCD44 and Ad-CD44S transduction on CD44 levels on day 7 in human trabecular meshwork cells. CD44 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and are expressed in ng/ml in both the adenoviral media and cell lysates. Fetal bovine serum (FBS) was used as negative (Neg) control; human serum (HS) was used as a positive (Pos) control. Data are the mean ± SEM; n=4. Analysis of variance (ANOVA) was used to determine significance between: Ad-Empty media versus Ad-sCD44 media ** p<0.01; Ad-Empty lysate versus Ad-sCD44 lysate, †† p<0.01; Ad-CD44S media versus Ad-sCD44 media, ‡‡ p<0.01; Ad-CD44S lysate versus Ad-sCD44 lysate, ‡‡‡ p<0.001; Ad-Empty lysate versus Ad-CD44S lysate, §§§ p<0.001.