Table 1 of
Yang, Mol Vis 2013; 19:986-994. Table 1 of
Yang, Mol Vis 2013; 19:986-994.
Table 1. Primer Sequence and Thermal Cycling Condition for Genotyping SALL4
| Amplicon | Forward Primer (5′ – 3′) | Reverse Primer (5′ – 3′) | Size (bp) | Ta (°C) | MgCl2 (mM) |
|---|---|---|---|---|---|
| 1 | TCAGGGCTCATGATAAATCG | AATCTCGGCTCCTGAATTTG | 402 | 58 | 2.0 |
| 2A | GATTATAGATGTGAGCGACGGTGC | CTTCCAGCTTTCTGGCTGAG | 795 | 60 | 1.5 |
| 2B | TACAGCAGATCCAGCTCACC | GCCACTTTGTCCTGGAACTC | 673 | 60 | 2.0 |
| 2C | TGGGACTGATAGCTCCTTGC | ACCCCAAGGTGTGTCTTCAG | 672 | 60 | 1.5 |
| 2D | TCAGAGCTCCCTCAAGATGC | CAGGCTCCTTTTTGATGACC | 791 | 58 | 2.0 |
| 3 | ACAAAGCCAGCTCCAGACTC | CGGCTTGTGCCAATAAGAAG | 471 | 60 | 2.0 |
| 4 | ATTCTTGGCTTGCCAGTGAG | TGTGTCTGCATTGCTCCTTC | 522 | 60 | 2.0 |