Figure 2. Retinal cells labeled with the lectin IB₄ from Griffonia simplicifolia (IB₄, green) in fresh neuroretinal samples (A) and experimental 9-day cultures (B–E). Ada: adalimumab treated culture; DAPI: 4’,6-diamino-2-phenylindole dihydrochloride staining (blue); INL: inner nuclear layer; GCL: ganglion cell layer; ONL: outer nuclear layer; TNFα: tumor necrosis factor alpha treated culture; TNFα/Ada: tumor necrosis factor alpha plus adalimumab treated culture. 4’,6-diamino-2-phenylindole dihydrochloride (DAPI) staining (blue) was present in the nuclei of the ganglion cell layer (GCL), the inner nuclear layer (INL), and the outer nuclear layer (ONL). IB₄-labeled cells were present between the INL and the GCL and extended into the INL in the fresh samples and the 9-day culture experiments (A–E, arrows). Scale bars: 20 µm.