Figure 7. Electromagnetic fields and retinal cells can affect the phosphorylation of ERK1/2, P38, Akt and JNK in human scleral fibroblast cells. Effect of exposure to ELF-EMFs on the phosphorylation of ERK1/2, P38, Akt and JNK in HFSFs treated with RPE supernatant or not. A: Western blot analysis was performed using p-ERK1/2, p-p38, ERK1/2, p38, and β-actin antibodies, respectively. B: Densitometric quantification of Phosphorylation levels were obtained by optical density (O.D.) of protein bands normalized with O.D. of β-actin band. Values (mean±SD, n=3) were expressed as relative expression levels. A quantitative analysis was performed by comparison with the untreated control. The double asterisk stands for p<0.01.