Figure 4. Electromagnetic fields and retinal cells can affect COL1A1 and MMP-2 protein expression in human scleral fibroblast cells. A: ELF-EMFs suppressed COL1A1 expression compared to control cells. Under the same radiation of ELF-EMFs, no significant difference was found in the expression of COL1A1 between the treated cells with RPE supernatant medium and the control cells. However, MMP-2 protein expression level was upregulated after treatment with ELF-EMFs and significant enhanced when treated with RPE supernatant medium under ELF-EMFs. B: Bar graphs show COL1A1, MMP-2 changes in protein expression (mean±SD, n=3) where density values were compared toβ-actin density. The asterisk indicates a significant difference relative to the control (p<0.05).