Figure 3. Electromagnetic fields can change COL1A1, MMP-2, TGFβ2, and FGF2 mRNA expression. COL1A1 and MMP-2 mRNA levels were measured with real-time PCR analysis after cells were treated with ELF-EMFs with or without RPE supernatant medium. mRNA abundance is expressed as cDNA copy number relative to copies of GAPDH. A: ELF-EMFs suppressed COL1A1 mRNA expression compared to control cells. After treatment with RPE supernatant medium, the decrease in COL1A1 mRNA levels induced by ELF-EMF exposure was accelerated. B: ELF-EMFs increased MMP-2 mRNA expression compared to control cells, and RPE supernatant medium induced ELF-EMF enhancement of MMP-2 expression. C: ELF-EMFs can affect TGFβ2 and FGF2 mRNA expression in RPE cells. Values (mean±standard error of the mean) were expressed as relative expression levels, the asterisk denotes p<0.05 and the double asterisk stands for p<0.01.