Table 1 of Puk, Mol Vis 2013; 19:877-884.

Table 1. List of PCR primers

Lab-No Sequence (5′ ->3′) Fragment size Annealing temperature
Pax6-L11 CTCACAGGCAGAAGACTTTAACC1 682 bp 53–64 °C3
Pax6-R11 CTTCCTGTTGCTGGCAGC1
Pax6-L12 CAACCTGGCTAGCGAAAAGC1 648 bp 53–64 °C3
Pax6-R12 TGCATAGGCAGGTTGTTTGC1
Pax6-L13 CTATCAGCAGCAGCTTCAGTACC1 616 bp 53–64 °C3
Pax6-R13 TTGTTCCAACTGATACCGTGC1
Pax6-Ex7-Int7-L1 ACAGAGTTCTTCGCAACCTGGC2 320 bp 67 °C4
Pax6-Ex7-Int7-R1 CCTTGACATACATAATCCTTACAGTCACC2
Pax6-Intron7-L2 TGTGAATCGGTGAGCTCTTAGACC2 410 bp 67 °C4
Pax6-Intron7-R2 TACATCAGAAGCCTGCACTGACC2

1the primers for PCR on cDNA are based upon the reference sequence BC036957.1 (GenBank); 2the primers for PCR on genomic DNA are based upon ENSMUST000000111086 (ENSEMBL); 3PCR was performed using Phusion-DNA Polymerase (Fermentas); 4PCR was performed using DreamTaq Polymerase (Fermentas).

Puk, Mol Vis 2013; 19:877-884. http://www.molvis.org/molvis/v19/877

©2013 Molecular Vision http://www.molvis.org/molvis/

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