Figure 4. Analysis of Aey80 genomic DNA. Sequence analysis of Pax6 intron 7 identified the 141 bp to be flanked by a G->T exchange four bases downstream of the insert sequence. Upper part: schematic drawing of the region of exons 7 and 8 including the intronic region; the location of the insert is indicated (not drawn to scale). Lower part: The four bases downstream of the new insert are given and particularly boxed in the sequences. Heterozygosity (R: G and A) is obvious in the heterozygous mutants; homozygous Aey80 mutants (embryos only) are given in the third row of sequences.