Figure 4 of Vincent, Mol Vis 2013; 19:852-860.

Figure 4. High-resolution melting analysis normalized difference graph of screening in the control population (200 alleles) for the visual system homeobox 1 gene c.731A>G, p.His244Arg heterozygous variant. The duplicate red lines running parallel to the x-axis at zero are the positive control (affected) samples, while the samples with no variants are represented by the sine-shaped melting curves.