Table 2 of Arranz-Valsero, Mol Vis 2013; 19:85-99.
Antibodies | Reference | Dilution | Time of incubation | Temperature (°C) | |
---|---|---|---|---|---|
IL-17RA | |||||
1st Ab | Rabbit anti-human | sc-30175 | WB 1:200 | Overnight | 4 |
IMF 1:50 | |||||
2nd Ab | Goat anti-rabbit | sc-2004 | WB 1:2000 | 1 h | RT |
Donkey anti-rabbit | A-21206 | IMF 1:100 | |||
α-Actinin | |||||
1st Ab | Mouse anti-human | sc-17829 | 1:500 | Overnight | 4 |
2nd Ab | Donkey anti-mouse | 715–035–150 | 1:5000 | 1 h | RT |