Table 1 of
Arranz-Valsero, Mol Vis 2013; 19:85-99. Table 1 of
Arranz-Valsero, Mol Vis 2013; 19:85-99.
Table 1. Gene primer pairs and amplification procedures.
| Gene | Primer sequence (5’-3’) | Amplification procedure | Length (bp) | ||
|---|---|---|---|---|---|
| Cycles | T (°C) | Time (s) | |||
| IL-6 | F:
GACAGCCACTCACCTCTTCA R: CATCCATCTTTTTCAGCCATC |
1 | 94 | 180 | 171 |
| 36 | 94 | 20 | |||
| 59.1 | 30 | ||||
| 72 | 30 | ||||
| IL-17A | Reference:
PPH00537B
Unknown sequence |
1 | 50 | 120 | Unknown |
| 1 | 95 | 600 | |||
| 40 | 95 | 15 | |||
| 60 | 60 | ||||
| IL-17RA | Reference:
PPH00983A
Unknown sequence |
1 | 50 | 120 | Unknown |
| 1 | 95 | 600 | |||
| 40 | 95 | 15 | |||
| 60 | 60 | ||||
| 18S | F:
ACTCAACAGGGGAAACCTCAGC
R: CGCTCCACCAACTAAGAACGG |
1 | 50 | 120 | 250 |
| 1 | 94 | 180 | |||
| 40 | 94 | 20 | |||
| 59 | 33 | ||||
| 72 | 40 | ||||