Table 1 of
Bennett, Mol Vis 2013; 19:835-844. Table 1 of
Bennett, Mol Vis 2013; 19:835-844.
Table 1. PCR primers used for amplification of human FTL and LIM2, and mouse FTL1.
| Primer | Location | Strand | Sequence (5′ - 3′) |
|---|---|---|---|
| FTL-IREF | 5′-UTR | sense | ATTTCACAACACGCTGGCGCTACA |
| FTL-Ex1R | Intron-1 | antisense | TCTGTTTACCCGACCGCACAAAGA |
| 32G>TR | IRE | antisense | GTTCCGTCCAAACACTGTTGAAGA |
| FTL-Ex2F | Intron-1 | sense | GAGTCCCCTTGGCCTCG |
| FTL-Ex2R | Intron-2 | antisense | GACACCTACGCCCTCAAATC |
| FTL-Ex3F | Intron-2 | sense | CCAACGACTCTTGGGAAATG |
| FTL-Ex3R | Intron-3 | antisense | AAAGGGAGCAGAGGCTTGAG |
| FTL-Ex4F | Intron-3 | sense | TCAGAGCCTCATTTCACACC |
| FTL-Ex4R | Exon-4 | antisense | CCAACTCCTCTTTCACTGGC |
| FTL-Start | Exon-1 | sense |
|
| FTL-Stop | Exon-4 | antisense |
|
| FTL1-Start | Exon-1 | sense |
|
| FTL1-Stop | Exon-4 | antisense |
|
| LIM2-Ex1F | Exon-1 | sense | GGACTTCCAGGTTCTGAGCAAGG |
| LIM2-Ex1R | Intron-1 | antisense | CGGACAAAGCCAGGGTTGCT |
| LIM2-Ex2F | Intron-1 | sense | TGTGCATGACACCTCTGAAGCG |
| LIM2-Ex2R | Intron-2 | antisense | GCATCCCACTCCTGAGCCCT |
| LIM2-Ex3F | Intron-2 | sense | GCTGAGGTGGAAGCAGTCTTGC |
| LIM2-Ex3R | Intron-3 | antisense | TTGCCAGGTGAAGAAGAGGGC |
| LIM2-Ex4F | Intron-3 | sense | CCCAACACCCTACTCTCTTTCTCCC |
| LIM2-Ex4R | Intron-4 | antisense | CCAAATATTGTTTCTCCCTCCCAGG |
| LIM2-Ex5F | Intron-4 | sense | CCACCCTTACAGCTGTTTCTCCC |
| LIM2-Ex5R | Exon-5 | antisense | CCCACATGAGTCCCACAGCA |