Table 1 of
Tiab, Mol Vis 2013; 19:829-834. Table 1 of
Tiab, Mol Vis 2013; 19:829-834.
Table 1. Primers for amplification of exons of CRB1
| Exons | Forward primer (5′ - 3′) | Reverse primer (5′ - 3′) | Annealing temp. (°C) |
|---|---|---|---|
| 1 | GTAGGGTGGGACAGAGATGG | AACTGACTGTTCACATTGACTGG | 60 |
| 2 | TTTTTCATTAGGATGAACCCAAC | CCATGTTGGCAGGAGTTCTT | 60 |
| 3 | CTCTGCCTTGTGCAACTTCC | TAAGCCGAGAACGTGAGAGC | 60 |
| 4 | CCATGGGTCTTGGGTTGATA | GCACCACAGCAGCAGAGTT | 60 |
| 5 | GATTCCCCTTACCAGCTCCT | GCACCACAGCAGCAGAGTT | 60 |
| 6a | AACCTGAGCTATTCATGCACTTC | TGGTGTTGTGGGAAATGAAC | 62 |
| 6b | GCAACAGGGATGTGTTTGTG | TTTCATAGCAGGCAGAAGCA | 60 |
| 7a | TTCGTCTTCCATCCCTTCTG | GTCAGGTAGGCCACCAATGT | 60 |
| 7b | GGAAATGTCCACTTGATATCTTTG | CTGGTGGGTCAGTAACATCATC | 60 |
| 8 | GCCCTTTTAGAAAGGAGTTGG | AGGCAAGAGGCCAGTCAGTA | 60 |
| 9a | CATGTATCAAATAGTCAATATGCAATG | GTCTGGGACAGTGGGTCTGT | 60 |
| 9b | ATTGCAAAGTGGCAACAGC | CAAGGGACAGGAGCAATGAT | 60 |
| 9c | CAGTGGTCACTGGCTGTTTG | CAGTGTCACCCTGTTCAGCA | 60 |
| 10 | CCTCCAGCAGGAGCTTTTTA | GCATAGATTTTCCTATGGGAACTG | 60 |
| 11 | AGACTGTGCTGTTCCAGAGAGA | TTTTTCATTTGGGAATGTTCAA | 60 |
| 12 | GCTTGCTCTGGTTGGTCTTC | TTTTAAGGTGGTCAAAGGAATCA | 60 |