Figure 1. Figure presents ELISA results for retinal Müller cells cultured in normal glucose (5 mM, NG) or high glucose (25 mM, HG) and treated with Compound 49b (50 nM) or PKA siRNA or CREB siRNA. A: These ELISA results for PKA activity demonstrate that PKA siRNA is effective in reducing PKA activity levels. B shows ELISA results for TNFα after Compound 49b treatment with or without CREB siRNA, to demonstrate that Compound 49b regulates TNFα through PKA and CREB action. *p<0.05 versus NG, #p<0.05 versus HG, $p<0.05 versus HG+49b. n=4 for all groups. Data are mean±SEM.