Figure 3. Insulin-like growth factor binding protein-3 (IGFBP-3) decreased intracellular adhesion molecule 1 (ICAM-1) expression in REC in high ambient glucose. ELISA results for IGFBP-3 (B) and ICAM-1 levels (A) in REC transfected with control or IGFBP-3 NB plasmid DNA for 24 h, following treatment with or without 10 ng/ml TNF-α for 4 h in high glucose (25 mM) medium. Equal protein was loaded for all samples, and data are presented as optical density measurements. *p<0.05 versus control plasmid only. ^#p<0.05 versus IGFBP-3 NB plasmid. ^$p<0.05 versus TNF-α. n=4, data are mean±SEM.