Figure 2 of Zhang, Mol Vis 2013; 19:796-803.


Figure 2. Insulin-like growth factor binding protein-3 (IGFBP-3) overexpression inhibited TNF-α and ICAM-1 expression in high glucose medium. For all panels, RECs were grown in 5 mM, 15 mM, or 25 mM glucose or 25 mM mannitol medium and transfected with either control or IGFBP-3NB plasmid DNA for 24 h. A: Results show IGFBP-3 levels using Western blot analyses to verify NIGFBP-3NB plasmid. B: Results of ICAM-1 ELISA for IGFBP-3 NB plasmid DNA transfection in REC in normal and high glucose. C: Results of TNF-α ELISA for IGFBP-3NB plasmid DNA transfection in REC in normal and high glucose. #p<0.05 versus 5 mM plasmid DNA; *p<0.05 versus control plasmid DNA. n=4, data are mean±SEM.