Figure 9. The activation of MIR146A promoter by proinflammatory cytokines in ARPE-19 cells was not affected by JAK inhibitor 1, while that of the MIR146B promoter was effectively blocked. The cells were transfected with promoter-luciferase constructs of MIR146A or MIR146B, and treated with a mixture of interferon (IFN)-γ (100 u/ml), tumor necrosis factor (TNF)-α (10 ng/ml), and interleukin (IL)-1β
(10 ng/ml) for 16 h in the presence or absence of JAK inhibitor 1. The promoter activity was estimated by measuring the luciferase
activity. *p<0.01 when compared to control, n=3; **p<0.01 when compared to cytokines, n=3. The data shown are representative
of three similar experiments.