Figure 6 of Kutty, Mol Vis 2013; 19:737-750.


Figure 6. The expression of miR-146a and miR-146b-5p is differentially regulated by interferon-γ, tumor necrosis factor-α, and interleukin-1β. Human retinal pigment epithelial (HRPE) cells were treated with the indicated cytokine or cytokine combination for 16 h. The concentrations of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β used were 100 u/ml, 10 ng/ml, and 10 ng/ml, respectively. The microRNA (miRNA) expression was analyzed by real-time PCR. A combination of all three cytokines elicited the highest increase in the expression of both miRNAs; however, miR-146a showed a dependency on IL-1β, while miR-146b-5p was dependent on IFN-γ. *p<0.001 when compared to control, n=4. The data shown are representative of three similar experiments.