Figure 4. The increase in the expression of miR-146a and miR-146b-5p in human retinal pigment epithelial cells by the inflammatory cytokines was time dependent. The cells were treated with a combination of interferon (IFN)-γ (100 u/ml), tumor necrosis factor (TNF)-α (10 ng/ml), and interleukin (IL)-1β (10 ng/ml) for the indicated time intervals and the microRNA (miRNA) expression was analyzed by real-time PCR. *p<0.001 when compared to 0 h, n=4. The data shown are representative of three separate experiments.