Figure 11. The expression of IRAK1 protein in ARPE-19 cells is decreased by miR-146a and miR-146b-5p mimics. The cells were transiently transfected with microRNA (miRNA) mimics for 72 h. A: Western immunoblot analysis of IRAK1 expression. Cells were treated with control miRNA mimic (random sequence), miR-146a mimic, or miR-146b-5p mimic. Actin was used as the loading control. A representative blot from three similar experiments is shown. B: Histogram showing IRAK1 expression. The IRAK1 band intensity was normalized with corresponding actin band intensity; *p<0.01 when compared to control, n=6.