Figure 5. Two-dimensional gel patterns of piscine lens proteins. (A) Zebrafish, (B) rice eel, and (C) catfish. Total protein (200 μg) in each sample was loaded onto immobilized pH gradient (IPG) gel strips (pH 3–10 Nonlinear, 13 cm). The procedures were as described previously [34]. After electrophoresis, the gels were fixed in 40% methanol and 10% acetic acid and stained by Coomassie blue G-250. The IPG strips were rehydrated, and after isoelectric focusing (IEF), subjected to two-dimensional (2D) electrophoresis. Protein spots marked with numbers were further identified by nano LC-MS/MS and listed in Table 2. The result is representative of three independent 2D experiments for each fish species. Identified crystallins by proteomic analysis were denoted by green (α-crystallin), black (β-crystallin), red (γ-crystallin), and yellow (noncrystallin proteins).