Figure 4. 1-anilinonaphthalene-8-sulfonic acid fluorescence spectra of recombinant wild-type and mutant transforming growth factor beta-induced protein. A: The 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectra of wild-type (WT), R124C, and R555W proteins incubated at 37 °C for various lengths of time. B: H₂O₂ (0.01%) was also added to the samples at the beginning of the incubation to investigate the effect of oxidation on the conformation of transforming growth factor beta-induced protein (TGFBIp).