Figure 3 of Grothe, Mol Vis 2013; 19:593-603.


Figure 3. Intrinsic tryptophan fluorescence of recombinant wild-type and mutant transforming growth factor beta-induced protein. A: The fluorescence spectra of recombinant transforming growth factor beta-induced protein (TGFBIp; 0.1 mg/ml) excited at 295 nm. The emission maxima are 329 nm for wild-type (WT) and 334 nm for R124C and R555W. B: The intrinsic fluorescence of recombinant TGFBIp was quenched using increasing concentrations of iodide (I-). The denaturation profiles of recombinant TGFBIp were investigated by (C) urea and (D) guanidine hydrochloride (GndHCl). The half-transition concentrations of GndHCl were determined to be 3.6±0.1 M for WT, 2.8±0.2 M for R124C, and 3.0±0.1 M R555W. (n=3, bar=standard deviation [SD]).