Figure 1. Distinct degradation patterns of recombinant wild-type and mutant transforming growth factor beta-induced protein. Recombinant (His)₆-TGFBIp (A) and (His)₆-TGFBIp-StrepII (B) proteins were produced by 293FT cells propagated in FreeStyle serum-free medium system, purified with Co²⁺-NTA chromatography, analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) gels and stained with Coomassie Brilliant Blue R-250. C: Wild-type (WT) and mutant proteins (0.1 mg/ml) from (B) were incubated at room temperature either with no protease added as the control, or with trypsin (1:50) or chymotrypsin (1:12,500) for 1 h and 24 h and analyzed with SDS–PAGE gels stained with Coomassie dye. Two μg of samples were loaded for each lane.