Figure 2. Pedigrees and zinc finger E-box binding homeobox 1 (ZEB1) sequences for the five families in which novel zinc finger E-box binding homeobox 1 mutations were identified. In each pedigree, the presence of the wild-type allele (designated by the + symbol) or the mutant allele is indicated below the symbol of each individual in whom DNA collection and zinc finger E-box binding homeobox 1 (ZEB1) gene screening were performed. Filled symbols represent affected individuals, open symbols represent unaffected individuals, and question marks indicate individuals of undetermined affected status. Arrowheads indicate probands. Beneath each pedigree, chromatograms demonstrating the identified mutation (MU) and the wild-type DNA sequence (WT) are shown.