Figure 6. Effect of B lymphoma Mo-MLV insertion 486 region 1 (BMI-1) on multicellular sphere formation of Y79 cells. A: Single transfected cells were cultured for 7 days to form multicellular spheres. The number and size of spheres were quantified. In triplicated experiments, the mean percentage of spheres having with diameters was indicated. B and C: Cell proliferation assay by BrdU incorporation for Y79 cells transfected with BMI-1-HA. B: BMI-1-HA and C: siBmi 1. Scale bar: 100 μm.