Figure 5. Influence of B lymphoma Mo-MLV insertion region 1 (BMI-1) on apoptosis and gene expression of Y79 cells. A: TUNEL assay showing increase of apoptosis rate after siBmi1 transfection to Y79 cells. A mild reduction of apoptosis was seen with BMI-1-HA expression (n=3, mean±standard deviation). *p<0.01; **p<0.005. B: Western blotting of pro- and active caspase-3 in Y79 cell lysate after transfection. Cells treated with camptothecin (10 μM) for 12 h served as the positive control of apoptosis. C: Propidium iodide cell cycle analysis of transfected Y79 cells by flow cytometry. D: Western blotting and band densitometry analysis of BMI-1, cyclin D1 and D2 in Y79 cells after transfection. *p<0.05, paired Student t test. E: Expression of CHX10, Rx, nestin, and neurofilament-M expression in transfected Y79 cells.