Figure 4. B lymphoma Mo-MLV insertion region 1 (BMI-1) expression altered retinoblastoma 480 Y79 cell growth. A-C: Immunostaining and reverse transcription (RT)–PCR analyses show marked increase of BMI-1 transcript and protein in Y79 cells after transfection with pCMV-HA/BMI-1 compared with vector control. D and E: MTT assay showing Y79 cell viability and proliferation was promoted by BMI-1 overexpression and reduced by siRNA-mediated inhibition of Bmi-1. F: Gene expression analysis of p14, p16, and PCNA in pCMV-HA/BMI-1, pSuper-BMI-1-si, and vector-transfected Y79 cells. *p<0.05, paired Student t test. Scale bar: 25 μm.