Figure 8. Curcumin attenuated hydrogen peroxide (H2O2)-induced expression of angiotensin II type 1 receptor, nuclear factor-kappa B, and vascular endothelial growth factor at
the mRNA (A) and protein (B) levels in ARPE-19 cells. The mRNA and protein measurements were done with quantitative real-time polymerase chain reaction
(qRT-PCR) and western blotting, respectively. Cells were treated with curcumin and hydrogen peroxide (H2O2) for 6 and 18 h, respectively. In the curc+H2O2 group, cells were treated with 6 h followed by insult with 200 μM H2O2 for 18 h; cells were washed with fresh media in-between the curcumin and H2O2 treatments. Representative western blots of angiotensin II type 1 receptor (AT1R), nuclear factor-kappa B (NF-κB), and actin, and densitometric analysis of the protein levels are shown in the upper and
lower panels, respectively (B). Data represent mean±SEM from three (for western) or four (for qRT-PCR) separate samples. *p<0.05 versus control, **p<0.001
versus control, #p<0.001 versus H2O2.