Figure 4. Curcumin attenuated hydrogen peroxide-induced microRNA expression in ARPE-19 cells. In the polymerase chain reaction (PCR)
array, cells were treated with 20 μM curcumin for 6 h and 200 μM hydrogen peroxide (H2O2) for 18 h. In the curcumin pretreatment group, the cells were treated with 20 μM curcumin for 6 h first, and after washing,
the cells were insulted with 200 μM H2O2 for 18 h. The curcumin pretreatment significantly suppressed the H2O2-induced expression of 17 microRNAs (miRNAs) compared to controls. Data represent mean±SEM from three separate samples. *p<0.05
versus control, **p<0.001 versus control, #p<0.001 versus H2O2, FC≥ or ≤2.