Figure 3 of Howell, Mol Vis 2013; 19:544-560.

Figure 3. Volcano plot of significance against the relative expression differences between the control and treated groups (AC). Each dot represents one of the 81 microRNAs (miRNAs) that was filtered and had detectable expression in either treatment. The X-axis displays log2-transformed signal intensity differences between the control group and the experimental group; the Y axis is the log-odds calculated according to the moderated Student t statistic test for differential expression between the control group and the treated group. The horizontal dashed line and the vertical lines represent significance threshold log-odds=2 and twofold expression differences, respectively. All spots above the horizontal dashed line are miRNAs that were identified as showing significant differential expression between the two treatments. MiRNAs positioned in the left and right upper-lateral quadrants represent downregulation and upregulation, respectively.