Figure 3. Inhibition of Vegf by SRPIN340. A: Bars represent real-time polymerase chain reaction (PCR) analysis of total Vegf mRNA. B: Bars represent real-time PCR analysis of exon 8a containing Vegf mRNA. C: ELISA of total VEGF protein in the RPE-choroid complex obtained from CNV mice 3 days after laser photocoagulation with intravitreal
injection of 0.1% DMSO or 20 pmol SRPIN340 (n=6 to 8 for real-time PCR analysis, n=8 to 10 for ELISA, respectively; n represents
the number of eyes). *, p<0.05; †, p<0.01.