Molecular Vision: Tea, Mol Vis 2013; 19:501-508. Table 2

Table 2. PCR primer amplification efficiencies of small ncRNA endogenous controls in rat liver.

Small ncRNA	Amplification efficiency in rat liver	R² value	Ct at 1/2 dilution
4.5S RNA(H) “Variant 1”	44.6%	0.99	25.4
4.5S RNA(H) “Variant 5”	ND – low abundance	ND	33.1
snoRNA202	ND – low abundance	ND	33.0
snoRNA	50.4%	1.00	26.9
Y1	ND – low abundance	ND	34.5

Five small ncRNA endogenous controls were tested to determine the PCR primer amplification efficiencies using total RNA derived from rat liver samples. Six serial twofold dilutions of the total RNA were used to generate the standard curves for 4.5S RNA (H) “Variant 1” and snoRNA. Cts were unable to be generated for all six twofold serial dilutions for the remaining small ncRNAs; therefore amplification efficiencies for these assays could not be determined. The R² value for each standard curve is shown, as is the mean Ct at a 1/2 dilution. ND=not able to be determined.