Figure 1. Sequence alignment of the FZD4 and LRP5 proteins. The FZD4 (upper panel) and LRP5 (lower panel) protein sequences of humans and other vertebrates were obtained from the UCSC Genome Browser and aligned against each other using the ClustalW software provided by EMBL-EBI, European Bioinformatics Institute [22]. Amino Acids are shown by one letter code and asterisks indicate the corresponding codons to nonsynonymous variants found in this study. Codons H69, R127 and Y211 of FZD4, and codons R1219, H1383 and T1540 of LRP5 are highly conserved in vertebrates.